Stable and destabilized GFP reporters to monitor calcineurin activity in Saccharomyces cerevisiae
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چکیده
منابع مشابه
Assays to Monitor Autophagy in Saccharomyces cerevisiae
Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is...
متن کاملCalcineurin-dependent Growth Control in Saccharomyces cerevisiae
Ca 2÷ ATPases deplete the cytosol of Ca 2÷ ions and are crucial to cellular Ca 2÷ homeostasis. The PMC1 gene of Saccharomyces cerevisiae encodes a vacuole membrane protein that is 40% identical to the plasma membrane Ca :÷ ATPases (PMCAs) of mammalian cells. Mutants lacking PMC1 grow well in standard media, but sequester Ca 2÷ into the vacuole at 20% of the wild-type levels, pmcl null mutants f...
متن کاملCalcineurin-dependent Growth Control in Saccharomyces cerevisiae
Ca 2÷ ATPases deplete the cytosol of Ca 2÷ ions and are crucial to cellular Ca 2÷ homeostasis. The PMC1 gene of Saccharomyces cerevisiae encodes a vacuole membrane protein that is 40% identical to the plasma membrane Ca :÷ ATPases (PMCAs) of mammalian cells. Mutants lacking PMC1 grow well in standard media, but sequester Ca 2÷ into the vacuole at 20% of the wild-type levels, pmcl null mutants f...
متن کاملCalcineurin-dependent Growth Control in Saccharomyces cerevisiae
Ca 2÷ ATPases deplete the cytosol of Ca 2÷ ions and are crucial to cellular Ca 2÷ homeostasis. The PMC1 gene of Saccharomyces cerevisiae encodes a vacuole membrane protein that is 40% identical to the plasma membrane Ca :÷ ATPases (PMCAs) of mammalian cells. Mutants lacking PMC1 grow well in standard media, but sequester Ca 2÷ into the vacuole at 20% of the wild-type levels, pmcl null mutants f...
متن کاملCalcineurin-dependent Growth Control in Saccharomyces cerevisiae
Ca 2÷ ATPases deplete the cytosol of Ca 2÷ ions and are crucial to cellular Ca 2÷ homeostasis. The PMC1 gene of Saccharomyces cerevisiae encodes a vacuole membrane protein that is 40% identical to the plasma membrane Ca :÷ ATPases (PMCAs) of mammalian cells. Mutants lacking PMC1 grow well in standard media, but sequester Ca 2÷ into the vacuole at 20% of the wild-type levels, pmcl null mutants f...
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ژورنال
عنوان ژورنال: Microbial Cell
سال: 2020
ISSN: 2311-2638
DOI: 10.15698/mic2020.04.713